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Improve Chromatographic Separations: Consider Mobile Phase pH & Analyte pKa

Chromatography aims to separate an analyte mixture into sharp, symmetrical, and well-resolved peaks for quantitative and qualitative analysis. To do this, it is crucial to understand ionization and the ionic form(s) of analytes at a given pH. The use of predictive software tools allows quick selection of optimal conditions and helps build robustness into methods from the start.
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